If we were to find the correct BIOS settings, it would be possible to use the x4 board instead." PCI Express Slot ExamplesĪdditionally, the power saving settings in the Bios need to be changed for similar strange power requirement reasons. It has become common practice to introduce energy saving modes into modern computers, and occasionally these can conflict with high performance devices. According to an Andor technician: "I believe that it might be due to some as yet unknown BIOS setting limiting the voltages to the x4 or lower PCI slots. However, it may need to be moved to a PCI express x8 or x16 slot not because of speed concerns but power issues. This card is normally installed in a PCI express x4 slot as it is supposed to be. The Andor Zyla camera is connected to the computer using a PCI express card x4 card. Specific Hardware Configuration for the Andor Zyla Camera Prior Lumen 200 Fluorescence Illumination System (Is not controlled directly by Micromanager, it's just a lamp) More Details.2 Lambda SC SmartShutters and Controllers (1 for DIC and 1 for Fluorescence ) More Details.Nikon TI Eclipse Microscope with Perfect Focus System (Includes a lot of components but you only need to worry about the scope itself) More Details.1.1 Specific Hardware Configuration for the Andor Zyla Cameraīefore beginning any setup, it is important to determine exactly all of the different components of the system.If not, I can also provide you with some screenshots. If you have problems finding the fields I indicated above, you might need to switch from beginner- to expert mode. if I remember correctlyīe aware, that you only need one of the three solutions. be aware that you must not add fr here, because this field has the unit attached automatically. Check this box and enter an appropriate value like 14990. make sure to always add the unit fr!!!)Ģ) you could also set a value for maxframe (change "expression to assign from" in an expression dropdown field to "maxframe" and then enter 14990 fr to the input field below)ģ) directly underneath the input field where you load the tif-files is a checkbox called something like "maximum number of frames" (don't remember the exact label but it shoud be easy to find). There are some ways to bypass this error - they all just skip the last frames of the measurement:ġ) set an expression filter like this: frame < 14990 fr (would skip the last 10 frames of a 15000 frames measurement. If I am right, fiji will tell you, that the file consists of, e.g., 14998 frames while it should have been 15000 frames. You can easily check this by, e.g., loading a "corrupted" file into fiji. It simply means that your data-acquisition software (I assume you use Andor Solis?) had some problems writing the last frames of your.
This is a very often encountered error but nothing you need to worry about too much.
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